In particular, I am studying the changes in the model that are observed after the induction or prevention of the nitric oxide signaling pathway, which is thought to play an important role in tumor migration. A key component of this pathway is inducible nitric oxide synthase (i.N.O.S.), an enzyme that produces nitric oxide (N.O.). It is thought that the overproduction of this enzyme in T.N.B.C. leads to more cell growth and movement. In previous work completed at CÚRAM, i.N.O.S. inhibitor and N.O. scavenger treatments were proven to reduce the migratory capabilities and viability of T.N.B.C. cells in 2-D. culture. Through the development of a T.N.B.C. spheroid model, it is our goal to prove the effectiveness of these treatments in 3-D.
